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Other mechanisms of controlling traffic across the BBB besides the structural support include ion channels and transport carriers, which control the traffic of hydrophilic nutrients, metabolites, vitamins and hormones, and ions across the BBB

doi: 10.1161/hypertensionaha.123.22649 72 McGarrahRWWhitePJ

Taking more than the recommended amount will not improve results and may cause adverse reactions

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The protein concentration was determined using the BCA Protein Assay Kit (Pioneer, Shanghai, China), and then the protein samples were transferred onto PVDF membranes by SDS-PAGE electrophoresis and then incubated with specific primary antibodies at 4 C overnight