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A 2024 study demonstrated that oral administration of low-dose ferroptosis inducers combined with anti-PD-1 therapy not only completely suppressed the primary colorectal tumor, but also conferred 100% immune protection in rechallenge models, highlighting its strong potential in inducing long-term memory responses (150)

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Then, cells were placed in a selective medium containing hygromycin B (100 g/mL for MCF-7 and 200 g/mL for SUM-159 and MDA-MB-231)

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