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doi: 10.1046/j.1600-079X.2003.00092.x 102

Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40

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The method provides reliable absorption, consistent dosing, and the highest tissue penetration