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After transfection for 48 h, cells were treated with metformin (1.0 mmol/L) for 2 h, followed by the PA exposure for additional 22 h

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While this suppression diminishes NF-B activation and subsequent pro-inflammatory mediator generation, irisin conversely activates the SIRT1-p53-SLC7A11/GPX4 axis to preserve redox balance and inhibit ferroptosis (116)

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Conversely, IDO1 and IL1B were identified as being downregulated 24 h after the immune challenge and subsequently upregulated