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Recalculate the concentration using the actual water volume you added and adjust your dose volume accordingly

Usually, the next step is to use mass spectrometry to identify those interacting proteins (Kassa et al, 2023)

This approach ensures that trace-level analytes remain sufficiently concentrated for reliable quantification, even though a less diluted sample inherently leads to higher matrix effects

Analysis of PB1 (IAV) transcripts was performed with SYBR Green-based qPCR using the following primers: forward: 5-ACACAAGGCCGACAGACCTA-3 and reverse: 5-ATGCTGTTGCAGCAGGTTGG-3 and Actb (QIAGEN

Withdraw the needle from the vial and gently swirl the vial until the powder is completely dissolved