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The EPCs on day 7 of the culture were transplanted to a 6-well plate (110 6 in 2 mL volume per well)

James takes ownership of the phrase, seeing it as an expression of the closeness and intimacy that a gay relationship can offer - something he was not exposed to growing up in the 1980s when there was a lack of positive representation of homosexual couples in mainstream media

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More powerful vapes produce more volatile compounds, they produce larger clouds of vapour that potentially impact those around you, and the number of replaceable parts means more things that can go wrong, more maintenance time and having spare parts on-hand to keep your vaping experience high-quality and safe