Digestion was completed by heating the samples to 90 C for 20 min, followed by dilution into an equal volume of hydrogen peroxide (30% Aristar BDH) and incubation for ~30 min period, allowing for effervescence to stop
Lin ZY, Wu Y, Xu G, Li Z, Li T, Liu
The term ROS encompasses a diverse array of oxidant molecules with varying properties and biological functions, ranging from cell signaling to causing cellular damage, as observed in the case of free radicals 44
The effect of pregnancy on subsequent relapse from Graves disease after a successful course of antithyroid drug therapy
For instance, even moderate amounts of folate can increase peripheral serum homocysteine, potentially inducing osteoblast apoptosis and stimulating osteoclast formation by promoting intracellular reactive oxygen species (ROS), hence contributing to OP (88, 89)