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This assay utilizes Ellmans method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB 2- )

But factors like aging, pollution, stress, and poor diet can deplete its levels

In vitro studies using normal human epidermal keratinocytes revealed that treatment with glutathione amino acid precursors significantly increased both GSH and glutathione disulfide levels, leading to an improved GSH/glutathione disulfide ratio, which is a key indicator of cellular redox balance

At moderate oxidative stress this effect small

Loren Pickart, PhD (2005) "Reverse Skin Aging: Using Your Skin's Natural Power