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Importantly, an additional experiment showed that LASSS-treated BSA (after the removal of un-reacted LASSS) exhibited nitration resistance to the optimal peroxynitrite-treatment (1:2000 ratio), as shown in supplementary Figure 2, suggesting a model that LASSS-induced chemical modification mechanism exerts a nitration-inhibitory effect on a wide range of proteins susceptible to tyrosine nitration
GSH/GSSG-Glo assay GSH/GSSG ratio of CuCl 2 -treated HepG2 and HepG2 ATP7B-KO cells was measured by GSH/GSSG-Glo Assay (Promega) according to manufactures instructions
Usually, yeah
L-Glutathione is found in every cell of the body
The wells were categorized into cell-free, untreated control, untreated control for cell lysis (serving as a control for maximum enzyme activity), and CGA-treated groups
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