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doi: 10.3390/ijms26125442 27 CuiC.TangJ.ChenJ.ZhangB.LiR.ZhangQ.et al

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RNA (1.8 g per sample) was reverse transcribed with RevertAid H minus first strand cDNA synthesis kit (Thermo Fisher, Waltham, USA) according to the manufacturers instructions in a total volume of 20 l, and cDNA was stored at 80 C until further use