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Immunohistochemistry was performed on floating sections using fluorescent dye-conjugated secondary antibodies, as previously described 69,70

Interestingly, DSIP is also found in human breast milk, where it is present in higher concentrations, suggesting a potential role in promoting sleep in neonates, highlighting its natural significance

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1) Biomarker challenges: Classical ferroptosis markers (MDA, 4-HNE, and C11-BODIPY) are non-specific, as they are also generated by other oxidative stress pathways (e.g., ROS from mitochondrial dysfunction)