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Our studies with LPS-induced Raw 264.7 cells demonstrated significant green fluorescence, indicating heightened HIF-1 expression and severe intracellular hypoxia (Fig

26, 27 Primary antibodies included anti-phospho-p65 antibody (Cell signaling #3033, 1:1000), anti-p65 antibody (Cell Signaling #6956, 1:1000), anti-IB antibody (Cell Signaling #3033, 1:1000), anti-IB antibody (Cell Signaling #9242, 1:1000), anti-phospho- IB antibody (Cell Signaling #6956, 1:2859) and anti--tubulin (Sigma-Aldrich #T-9026, 1:5000)

The column was washed with acetonitrile for 10 min followed by a linear gradient back to the initial mixture of 30% acetonitrile with the flow rate 1 ml/min

Its protective role can improve overall health by ensuring good liver function

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