In: Zeigler-Hill V, Shackelford TK, eds
The active ingredient was identical
The same trained evaluator assessed all subjects under controlled conditions at both baseline and Week 12 ensuring consistent, unbiased scoring
Cellular adhesions marked by endogenous paxillin-GFP were labeled with anti-GFP primary antibodies (Life Technologies, Carlsbad, CA, USA, Cat# A-11122, RRID:AB_221569, dilution: 1:500) and Anti-Rabbit Alexa Fluor 488 secondary antibodies (Invitrogen/Fisher Scientific, Goat Polyclonal, Cat# A-11008, RRID:AB_143165, dilution: 1:500) in the m-EGFP-tagged Paxillin (PXN) WTC iPSC line, while the actin cytoskeleton was labeled with Acti-stain 488 (Cytoskeleton, Inc., Denver, CO, USA, Cat# PHDG1-A) in the TagBFP-tagged d-Cas9-KRAB WTC iPSC lines
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