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For mKate reporter construction, the mKate2 gene was amplified from pRN10 (Addgene, plasmid 84454) 69 using a specific primer pair (Supplementary Table 3) and Q5 High-Fidelity DNA Polymerase (New England Biolabs)

Key Research Areas These areas reflect ongoing scientific investigations and are provided strictly for laboratory context

After the February 2025 shortage resolution and April 22, 2025 enforcement deadline, each prescription requires prescriber evaluation and documented clinical rationale not just shortage status reasoning

To create this texture, I first use a light blue as the base color

One of those pathways is inflammation