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After centrifugation for 10 min at 4 C, the supernatant was transferred to analytical vials and stored at -80 C until LC-MS analysis

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Head-to-head comparisons identified the combination of the mPGK promoter (P mPGK ) driving MTNR1A and the pVH421 reporter as the most effective, yielding the highest SEAP expression and robust fold induction in response to melatonin (Fig

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