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After undergoing three times of PBS washing, the cells were subjected to incubation in a staining solution comprising 5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT, Beyotime, China) for a duration of 30 min at room temperature under light-free conditions

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10.1074/jbc.M115.679514 J

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