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Biochemical analyses Transfected N2a cells were lysed with ice-cold Lysis buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM MgCl 2 , 2 mM EDTA, 1% Triton X-100, 0.1% SDS, and 10% glycerol) supplemented with a protease inhibitor cocktail (Roche)

the evidence base is still developing, so we frame this as a mechanism under investigation rather than a settled outcome

Linda Bluestein: And are there any red flags that people should be aware of as they're rehabilitating themselves that say, ooh, you better pause right away

Metabotropic glutamate receptor 5 and glutamate involvement in major depressive disorder: a multimodal imaging study

Jutley GS, Rajaratnam R, Halpern J, Salim A, Emmett C