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The membrane was blocked for 2 h with 5% nonfat dry milk in TBS-Tween (TBS-T: 50 mM Tris-HCl, 150 mm NaCl, and 0.1% Tween 20 (pH 7.4)), and then incubated overnight at 4 C with 1.78 g/ml GLP1R-specific antibody (MAb 3F52) 24 in TBS-T

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Taurines antioxidant properties can reduce oxidative stress, potentially slowing the aging process and protecting against various diseases

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The animal literature is unusually consistent, though: nearly every published in vivo study used 5 mg/kg per injection, given three times on alternate days (days 1, 3, and 5, or days 14, 16, and 18 in the pulmonary fibrosis model), with effects tracked over the following three to four weeks