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This assay operates on the principle that peroxides oxidize ferrous ions (Fe 2+ ) to ferric ions (Fe 3+ ) under acidic conditions

D., & Thalib, L

Furthermore, BP samples of three stingless bees, i.e., Heterotrigona itama, Tetrigona apicalis, and Geniotrigona thoracica (from Malaysia) were tested in vitro upon MCF-7 (breast adenocarcinoma human cell lines) and MCF-10A (mammary epithelial human cell lines), which inhibits the proliferation of both tumor cell lines in a dose-dependent way (181)

Such a setup would precisely delineate the NSC-derived paracrine factors (e.g., BDNF, VEGF [18, 32], TGF-) responsible for suppressing microglial activation and promoting an anti-inflammatory phenotype, independently of in vivo complexities