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Arabidopsis cotyledons from two independent eYFP-GLYR1 transgenic lines were imaged using a Leica Stellaris 5 confocal laser scanning microscope with 63X oil objective (NA: 1.40)

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For the quantification of prefrontal GABA levels, a Mescher-Garwood Point Resolved Spectroscopy (MEGA-PRESS) sequence was employed, utilizing a simultaneous spectral editing technique