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Cell lysates were boiled for 15 min and protein concentration was determined by the Lowry method

And then, RNA was sequenced on Illumina HiSeq 2500 for 125 bp paired-end reads at Biomarker Biotechnology Corporation (Beijing, China)

Allicin promotes functional recovery in ischemic stroke via glutathione peroxidase-1 activation of Src-Akt-Erk

Statistical analysis All data were presented as mean standard deviation (SD) from at least three independent experiments unless otherwise stated, and concentration response curves were plotted using GraphPad Prism software