Harvested cells were resuspended in lysis buffer (20 mM HEPES-NaOH pH 7.4, 500 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP, 1 mM PMSF), then lysed by sonication
Selim H, Shaheen S, Barakat K, Selim AA
It is generally stored refrigerated at 2 to 8C and protected from light
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These can be signs of an infection or a total blockage, which require urgent care
