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After tumor development, the siGGCT group received peritumoral injections of NAC or physiological saline, and the tumors were weighed every five days until the samples were harvested for final analysis (Fig

This involved adding a lipid-ethanol solution to a plasmid solution in 25 mM citrate buffer (pH 4.0), followed by buffer replacement with PBS through diafiltration using Amicon Ultra Centrifuga Filter (10 kDa MWCO, Millipore)