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PRMT1 regulates fatty acid metabolism by methylating UBE2m at R169 in TECs Next, we predicted the position of UBE2m arginine methylation by using the GPS-MSP tool [33], among which R169 had the highest methylation score (Fig

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In brief, 500 cells were seeded per well in 384-well plates, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium