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Generation of Cyclic AMP in Taste Buds of the Rat Circumvallate Papilla in Response to Sucrose

Simply freeze the cooling block overnight and insert into thermal protection sleeve

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To measure PIP 3 , FoB were stimulated with 5 g/mL anti-IgM (Jackson Immunoresearch 715-006-020) for the indicated time points, then fixed in 2% FA and permeabilized with 0.1% saponin