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Specifically, we showed that SLC25A45 mediates the mitochondrial import of TML across the IMM, which is required for de novo carnitine synthesis in vivo ( Figure 7 )

These studies confirmed that GABA release is dependent on the entry of extracellular Ca 2+ through voltage-gated channels and not by membrane depolarization itself

Site-directed mutagenesis Mutant forms of mito GCL refractory to the shRNA against GCL ( mito GCLmut) or catalytically inactive mito GCL ( mito GCL (E103A)) were generated using the site-directed mutagenesis QuikChange XL kit (Stratagene, La Jolla, CA, USA), followed by Dpn I digestion

Normalised probeset expression measures were calculated using log 2 transformation and quantile normalisation using the Lumi package

Assisting physical stress