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To support the hypothesis that hiPSCs-derived MVs contain wound-healing messengers, first we investigated some important mRNAs inside the hiPSCs-derived MVs, including AQP3, COL2A, FGF2, ITGB, and SEPTIN4, that involve in wound-healing mechanisms seriously 8,9,10,11,12,13,14
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Running them together under a prescribed protocol covers both layers of the healing process
For the electrophysiological measurements, cell confluence ranged between 60 and 80%