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After the initial baseline challenge, subjects were placed on a week-long oral regimen of a cocktail of poorly absorbed antibiotics previously shown to effectively suppress intestinal microbiota (6), and then the d 3 -l-carnitine challenge was repeated (Figure 1, open circles)

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doi: 10.1097/GIM

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Animal LAD and MCAO experiments For LAD and MCAO experiments, male C57BL/6 J mice aged 812 weeks were ordered from Charles River Laboratories UK (Margate, UK) and were housed in individually ventilated cages and maintained under controlled temperature (22 2 C) on a 12 h light/dark cycle with ad libitum access to chow and water