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Tagging the protein of interest to Trx is a widely used method to reduce inclusion body formation, and a previous study from our lab reported an improvement in soluble expression in E

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The pellet was re-suspended in 12 mL lysis buffer (25 mM HEPES p H 7.5, 100 mM NaCl, 5% (w/v) glycerol, 5 mM GSH)

The ligand, OPGL, also known as RANKL, TRANCE, or osteoclast differentiation factor (ODF), is a transmembrane protein expressed by osteoblasts and found in several organs (241)