To explore the underlying mechanism of SP@M in alleviating RA, the synovium and proliferated inflammation tissue around the joints in upper limbs in the saline group and SP@M group were isolated, respectively, for RNA sequencing analysis (Supplementary Fig
The GSSG-dependent oxidation of roGFP2 by fused PfGrx variants is two to three orders of magnitude faster than the oxidation by unfused PfGrx(SSG) variants and more than seven orders of magnitude faster than the uncatalyzed reaction with GSSG (Table 1)
Reconstitution 3-step visual Reconstitute , add bacteriostatic water down the wall of the vial Measure , use the calculator (section 8) to compute the required volume Store , refrigerator 2 to 8 C, strictly protect from light Detailed protocol What you will need: A vial of GHK-Cu (50 mg blue lyophilizate) 5 ml of bacteriostatic water (0.9 % benzyl alcohol) Insulin syringe 1 ml / 29G Dim work environment (if possible) Procedure: Allow the vial of GHK-Cu to reach room temperature (15 to 20 min)
Incubate at 37 C for 60 min, protected from light
This maintains effectiveness while reducing potential for excess accumulation
