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studied the anti-mutagenic effect of low dose (30 nM) selenite-induced GPx activity against -irradiation (8 Gy) by monitoring the mutation frequency of the hprt locus in a panel of four cell lines (NIH3T3, PC12, MCF7, Sup-T1, and AA8) (Table 2) [48, 49]

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However, the reliability of this technique is undermined by the formation of GST-fused protein aggregates after incubation with cell lysates