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IR flox/flox mice, initially generated from C57BL/6J and CD-1 background, had been backcrossed for at least five generations on a C57BL6 background, and Col2-Cre mice, initially established on a C57BL6/129sv background, had been backcrossed for 10 generations on a C57BL6 background before being intercrossed with IR flox/flox mice

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This strong connection allows the vii chord to function as a substitute for the dominant, creating a very similar and powerful pull towards the tonic, C Major