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Slides were kept at 37C in a gaseous mixture consisting of 3% O 2 , 3% CO 2 , and 94% N 2 for up to 30 min and then immediately used for microscopy not longer than 30 (usually only 5) min

Microbiota metabolite short-chain fatty acid acetate promotes intestinal IgA response to microbiota which is mediated by GPR43

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A., Davis R., et al., Exendin4 Reduces Fasting and Postprandial Glucose and Decreases Energy Intake in Healthy Volunteers, American Journal of PhysiologyEndocrinology And Metabolism 281 (2001): E155E161, 10.1152/ajpendo.2001.281.1.e155