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This mechanism is critical because neurons have a limited ability to synthesize endogenous GS ( Oxidative stress in the CNS acts as a persistent inflammatory trigger by activating brain-resident immune cells, particularly microglia and astrocytes

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GSH concentrations were determined using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system, specifically the UltiMate 3000 series coupled with the TSQ Endura Triple Quadrupole Mass Spectrometer