To determine the likelihood of SyeA orthologues from diverse Buchnera being substrates for a T3SS, a total of 103 SyeA orthologues was run in BastionX, with the common use parameter for v.2.0 selected (see Supplementary Dataset 2 at Figshare 17 )
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This process avoids adding external molecules since interferon gamma (IFN-) and tumor necrosis factor-alpha (TNF-), two cytokines associated to the SASP, act as natural adjuvant by promoting an immunoreaction