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147 published a randomized trial in which the melatonin/hypothermia group, at 5 days of life, had fewer seizures and fewer white matter abnormalities on MRI

For the untargeted experiment, proteins were digested with 0.7 g of trypsin (sequencing grade, Promega, Madison, WI) and 0.7 g of Lys-C (New England Biolabs) for 1 h at 37 C

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