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Wild-type enzyme and K105X mutants tended to have infinite true k cat and K m values for extrapolated infinite substrate concentrations, suggesting that the enzymes are neither saturated by GSSCys nor GSH (Supplementary Fig

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The three amino acids cysteine, glutamate, and glycine combine to form the tripeptide glutathione

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Paracetamol poisoning occurs especially in small children who have low hepatic glucuronide conjugating abil ity