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After 8 h, cells were harvested in Passive Lysis Buffer (Promega Corp., Madison, WI) and the luciferase activity was measured by the Nano Dual-luciferase report assay System (Promega Corp., Madison, WI)
Human data are limited and primarily from aging studies rather than fertility outcomes
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Antioxidant therapies that specifically target mitochondria, such as coenzyme Q10 and mitochondrial-targeted antioxidants (e.g., mitoquinone), have shown promise in reducing mitochondrial oxidative stress and improving sperm motility (93)
, 60 (Suppl 5), 7176
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