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Bifrontal electrode placement in ECT has been associated with faster response and fewer cognitive adverse effects compared to bitemporal placement.[24,25] Third-line options for acute mania with more limited evidence include monotherapy or combination therapy with chlorpromazine or clozapine, and combination treatment with haloperidol and carbamazepine or oxcarbazepine, or other combinations of first-line agents not tried earlier

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HPLC/mass spectrometry quantification of 8-oxo-dG DNA was extracted from brain tissues using standard phenol chloroform methodology for which the methodological details have been published previously