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Endocrinology 146 , 51205127 (2005)
Essential Fact #3: Specialized Dosing for Chronic Conditions Managing vitamin B12 deficiency in chronic conditions needs special dosing
This was followed by transduction with iCas9 and guide expressing vectors (Control and sg Gstt1 ), harvesting by trypsinization, washing twice in PBS, resuspension at 1 x 10 4 in 90ul PBS and 10% Matrigel (Corning), and injected into the 4 th inguinal mammary fat pad of BALB/c mice (Jackson Laboratory) to establish mammary tumors
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