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Beyond histological changes, both regions displayed a consistent enteric nervous system imbalance, characterised by a dose-dependent reduction in neurons expressing neuronal nitric oxide synthase (nNOS), cocaine- and amphetamine-regulated transcript (CART), vesicular acetylcholine transporter (VAChT) and vasoactive intestinal peptide (VIP), and a corresponding increase in galanin and substance P neurons, indicating a shift toward a pro-inflammatory neuronal profile