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Indeed, in regular firing interneurons from CA1 region of the hippocampus, LTP could be induced by trains of postsynaptic spikes (about 600 APs) evoked by depolarizing pulses without presynaptic activity (Nicholson and Kullmann, 2+ ] i rise, VGCCs, and release from internal stores were activated by the AP-only protocol sufficiently strong to produce calcium levels necessary for triggering plasticity all over the dendritic tree

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The protein sample was extracted from membrane by adding a 2 solubilization buffer containing 20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) n-dodecyl-beta-D-maltopyranoside (DDM, Affymetrix), 0.4% (w/v) cholesteryl hemisuccinate (CHS, Sigma), and 2% (w/v) glycerol for 3 h at 4 C