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[NCBI] Song, Ran & Choi, Hyun & Yang, Hyung-In & Yoo, Myung & Park, Yong-Beom & Kim, Kyoung

100mg (Passiflora incarnata) (flower) (standardized to 3.5% flavonoids) L-Theanine

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

This enzyme has the task of combining the amino acids glutamate and cysteine to form gamma-glutamylcysteine (GGC), which is used to produce glutathione (GSH) by the GS enzyme

From these five studies, in two, in vivo delivery into the target tissue, brain or brain tumor, was evaluated in any capacity