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Statistical significance between pairs of groups was assessed using an unpaired two-sided t-test, one-way ANOVA followed by the Dunnett multiple comparison test, or two-way ANOVA with the Tukey multiple comparison test, as indicated

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Equal amounts of proteins from lysates were separated by SDSPAGE using Mini-PROTEAN TGX 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad)

I had seen a number of anecdotal reports, case reports, and literature describing similar symptoms as this particular patient