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Protein Gantibody/chromatin complexes, as well as the 2% input solution, were eluted with 150 l 1 ChIP elution buffer two times, and recovered by adding 5 M NaCl and 100 g/ml protease K and incubating 2 h at 65C
Reactions were incubated for 30 min at room temperature in white, clear-bottom microtiter plates, and per cent of the maximal response was calculated using control wells as a reference
We have a provider group that's trained on how to prescribe the oral medications, and they're going to be trained on how to prescribe the GLP-1s," Carroll said