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Most tested conditions fit the linear region of the standard curve, but for BDNF, a few samples diluted at 1:20 and 1:40 in the human free BDNF Quantikine ELISA kit (#DBD00, R&D Systems) and in the high-sensitivity BDNF ELISA kit (#SK00752-01, Aviscera Bioscience), respectively, were above the highest declared point