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after reduction and alkylation using DTT and IAA, the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius), washed twice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and twice with 50 mM ammoniumbicarbonate

This is undoubtedly a step forward in ovarian cancer research, as the late diagnosis is one of the most important barriers accounting for the poor outcome and high mortality

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If oral B12 replacement has been trialled in a person with metformin-associated B12 deficiency, I would suggest that a repeat measurement of B12 levels after 812 weeks is a good time to reassess whether absorption is adequate or whether a switch to intramuscular replacement is required

2021;162 doi: 10.1210/endocr/bqaa246