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In parallel with these changes, a decrease in the activity of antioxidant enzymes (GPx, CAT, GR, SOD) and GSH was noted ( 4.1 Oxidative stress in HT There are a growing number of reports showing that increased oxidative stress pathways lead to increased synthesis and release of a wide variety of immune and inflammatory molecules

ROS quantification was performed on a Spark Multimode Microplate Reader (Tecan Life Sciences, Mannedorf, Switzerland) at an Ex/Em of 485/535 hourly for 8 h

Lau J, Ioannidis JPA, Schmid CH

Methods Preparation of the ASAP1 PH domain The sequence for mouse ASAP1 PH domain, [325451]-ASAP1, was cloned between Nde I and Bam HI restriction sites of the pET3a vector, which was then transformed into Escherichia coli BL21 Star (DE3) cells (Invitrogen) for protein expression, plated on LB agar containing carbenicillin (100 mg/L), and incubated overnight (o/n)

manufacturing to keep up with demand