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Such a step applies in refrigerator settings

Ethyl acetate, ethylenediamineteraacetic acid, glutamic acid, n-butanol, nicotinamide adenine dinucleotide, nitro blue tetrazolium, N-naphthyl ethylene diamine, pilocarpine, picrotoxin, pyridine, pyridoxal phosphate, sodium dodecyl sulphate, sodium chloride, sulfanilamide, thiobarbituric acid, tris-HCl, -oxoglutarate and 5'5-dithiobis (2-nitrobenzoic acid), and all the other reagents used for biochemical analyses were purchased from Sigma Chemical-Corporation, USA

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