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Tumor diameters were measured with digital calipers at multiple time points (days 3, 5, 7, 9, and 11), and the tumor area (in mm 2 ) was calculated with the following formula: area = 3.14 width length/4
The intervention also suppresses opportunistic pathogens ( Plesiomonas , Aeromonas , Shewanella ) in a dose-dependent manner, with microbiota shifts coinciding with enhanced hepatic antioxidant enzyme activities
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